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By V. L. Yarovenko (auth.)

ISBN-10: 0387086064

ISBN-13: 9780387086064

ISBN-10: 3540086064

ISBN-13: 9783540086062

ISBN-10: 3540359508

ISBN-13: 9783540359500

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45] for Candida intermedia IFO 0761 as an example. 1 Transfer of Substrate During Clump Formation Accompanied Growth Microorganisms with high affinity for liquid hydrocarbon often form clumps with hydrocarbon drops during growth (Mimura et al. [ 1], Blanch and Einsele [2]; Einsele et al. [31 ] and Nakahara et al. [33]). C. intermedia IFO 0761 adhers tightly to the surface of the hydrocarbon drop and the microbes on the hydrocarbon drops form clumps during growth, as shown in Section 2. The transfer of substrates other than hydrocarbon was investigated in the growth which accompanied clump formation.

The formation of the mannan-fatty acid-complex was studied during a substrate shift from glucose to hydrocarbons. The decrease of affinity on glucose is considered to be a consequence of a diminished lipophility at the cell surface. The porous structure of the cell surface layer is considered not to be affected by the substrate change. An emulsifying agent which was homologous to the mannan-fatty acid-complex at the cell surface, was isolated from the medium. Mechanism of Liquid Hydrocarbon Uptake by Microorganisms and Growth Kinetics 33 Table 1.

2 Adhesive Force . . . . . . . . . . . . . . . . . . . . 3. Pathway for Liquid Hydrocarbon Uptake . . . . . . . . . . . . . . 4. Hydrocarbon Pool . . . . . . . . . . . . . . . . . . . . . 5. Growth Kinetics of Microorganisms with Low Affinity for Liquid Hydrocarbon . . . 6. Growth Kinetics of Microorganisms with High Affinity for Liquid Hydrocarbon . . . 1 Transfer of Substrate During Clump Formation Accompanied Growth .

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Advances in Biochemical Engineering, Volume 9 by V. L. Yarovenko (auth.)


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